Inside the issue - which is available for just £1.99 on your iPad - Johansson discusses the differences between the first film and the sequel, directed by Joe and Anthony Russo.
"I don't spent a lot of time in my catsuit in this one," she says. "I take it to the streets in a different way.
"There's a real kind of Big Brother aspect to this film. All that Cap and Widow know to be true, everything that they trust in – as much as they trust in anything – is sort of ripped out from underneath them."
Captain America: The Winter Soldier opens in the UK on 28 March 2014.
For more from Johansson, pick up the brand new issue of Total Film , which is out now , and includes an exclusive look at 300: Rise Of An Empire , X-Men: Days Of Future Past and Godzilla , plus many more exciting new movies.