Gino - The Darkness
Notable for: Having one of the earliest and most hilariously unexpected deaths in videogames. Teaching us why we shouldn't lean out of moving cars. Trucks are heavy, kids.
Chewed up Pikmin ghosts - Pikmin
Notable for: Teaching us that even sentient vegetable people have souls. Making us feel guilty and creeped out at the same time.
Murdered Colossi - Shadow of the Colossus
Notable for: Making us feel like total wankers as we turn a once proud and noble creature into just another pile of rock and mould.
Skulls - Zelda III: A Link to the Past
Notable for: Being one of the first instances in which we could use a piece of disembodied anatomy as a tool or weapon in a game. Speaking of which...
McCoy and the crazy nurse - Dead Space
Notable for: Proving that love can blossom between two people even if one of them is a dismembered corpse. Especially if one of them is blind and insane. Providing endless squishy fun when stomped on. Confirming that there's one thing a gamer will always do when presented with body parts and a gravity gun.
Giant slug-fish thing - Metroid Prime
Notable for: Being the first of many dead things in MP we could investigate with the scan visor. Training us to poke around in corpses for educational value.
Aeris - Final Fantasy VII
Notable for: Still being the subject of fanboy mourning 11 years later. Get over it! Please!